Isnin, 12 Disember 2016

GAHARU MICROBIOLOGY.. Scientific Expression.

I edit this record back and restored as the main study the formation of agar wood through technology. Naturally I'm really interested in studying the pathogenesis, biotechnology, microbiology, oleoresin and sesquiterpenes biosynthesis. It is a field that is parallel or related processes and production of natural sandalwood through technology. Although it is quite difficult to explain in writing, it is the expressions, terms according to a scientific word. At least we understand, aloes produced and formed as infected with fungus species, from the information I collect articles derived from 22 previous studies that had found.


Sometimes I like to invite you to appreciate while reading blog posts sandalwood me, I described you as being under sandalwood, as far as you can imagine, how the work of inoculation that we do, how to notice you when you are above the tree 4 of adherents with a height of 40 feet with drill in hand, how the sense of fatigue experienced when transporting equipment inoculation. Jungle trekking and climbing hills and how a sense of satisfaction after inoculation performed within 3 months.


After a few notes topic I built on the species of fungus, pathology, biotechnology, microbiology, oleoresin and sesquiterpenes biosynthesis, my goal, to reveal expressions, terms according to the word of scientific, because I am involved in this field, or in the field of sandalwood technology and not natural sandalwood, but not means I not take it lightly against natural sandalwood, due to difficulty in obtaining natural sandalwood, shortcut I had been active in the field of technology sandalwood.


Aquilaria malaccensis Lamk (or sink, sandalwood or depu elephant, family Thymalacceaceae) between sandalwood species are capable of producing oleoresin woody fragrance called "order" (sandalwood / agar wood) Kainan equivalent grade. To be considered as a pathological product produced by species of fungus attacks wood-decaying fungi, oleoresin act as a regulator. (Bhattacharyya 1952 / Kamuli 2000). Between different fungal species associated with that zone, may exhibit patologenesis while others seem saprophytic, one of the symptoms of diseases of fungal attack for no tissues around the tree is injured or hurt.


Changes in the pathogenesis of Cellulolytic and pectinolytic activities have been reported (Prassad 1988 & Reddy1988). The increase in peroxidase oxidase activity in plant tissue following infection by a wide variety of pathogens have been reported by researchers (Loebenstein & Linsey 1961: / Addy & Goodman 1972). The study has been conducted to study the changes celulase, pectinase, peroxidase and polyphenol oxidase activity during the Pathogenesis in agar wood inoculation by injection isolates of the fungus, the fungus isolated them (Chaetomium globosum Kunze and Fusarium oxysporum shiecht) The injection into a combination of a healthy, aimed at stimulating the breeding of fungus in trunk.


Enzyme activity was measured in a healthy condition, naturally and artificially infected samples inoculated plants, how intervals of 10, 20, 30 and 40 days after inoculation. The aim is to estimate celulase events and activities in accordance with methods and special activities have been adopting as a study (Verma and Singh 1975). Pectinase activity while the reaction mixture of 1 Pertin checkers 1mL enymemeng extract. (Dubey & Mathur, 1975).


One unit of enzyme activity is stated lose 1% of the concentration of 30 min, peroxidase was extracted through methods by three researchers (Chakravarti & Nadi Purohit 1978 and 1979). Activities polyphenol oxidase method discovered by Purohit 1979 determining catechol is used as the substrate and pectinase activity. Maximum enzyme activity was recorded in plants 20 days after inoculation, however, reduced to 40 days, and a pectinase specific activity celluase is highest are in natural wood in infected, compared to healthy wood.


Peroxidase activity in healthy samples remained unchanged 40 days of incubation. Specific activities listed in the 0077 natural wood that has been infected and 0052 were found in healthy wood. Polyphenol oxidase activity was found in a sample of healthy wood, does not have a significant increase during the incubation, however, a substantial increase in the sample polyphenol oxidase activity increased during the incubation process.

The maximum specific activity is 0,119 on the infected wood and wood stays healthy 0,054, review all investigations showed activity of all enzymes to 20 days incubation process, then on days 30 to 40 days of incubation, the activities of both enzymes decreased. Therefore, celulase higher and pectinase activity was observed in the infected wood, likely responsible no wood around the tissue leading to colonization of pathogens and changes in enzyme seeds. (Prasad 1988).


Multi-range study found that the enzyme activity of pectolytic and Cellulolytic enhanced due to the effect of Aspergillus flavus, proposed that a higher percentage of enzymatic pectolytic and Cellulolytic used as a tool for penetration fungi, because the enzyme responsible could impair the cell, increasing the activity of enzymes in tissues compared healthy tissue, caused by pathogenic events, activities and polifenoloxidase Proxidane equally rose together through the incubation process during treatment. (Reddy 1988).


However. Maximize enzyme activity, have been recorded as a natural sample infected and healthy sample. Peroxidase and polyphenol oxidase activity is in the leaves inoculated with Erwiniaa mylovora which has been studied by Addy & Goodman, 1972. Through the arguments expressed in this study, increased activity of both enzymes after being injected impinge as controller, suggesting higher utilization peroxidase in plants infected, probably due to greater damage through monophosphate carbs, phenolic compounds produced an oxidase (H202) to overcome Pathogen. (Loebenstein & Linsey 1980).


Member spots researchers agree the main products produce sandalwood caused pathological established as a result of a fungal infection, but there is no role of any fungus given no relevant formation (oleoresin) aloes, some say sandalwood formed by the tree in response to Ramesh (Jamaluddin 1977, Rahman & Khisa 1984, Gibson Chalempongse 1877 and 1990).


We change the terms of sandalwood produced because the hurt, the wood is infected (fungi) resulting from activities mikrod and pathology, no one can be angry too, we change the terms of that, it is true, according to reports and studies researchers before, unanimously said aloes resulting from the infection, over and over again, galore provided under Article notes the report of the survey they touched on how the wood in infected produce sandalwood, but still there is a conflict theories and opinions about reproductive related species of fungus, although studies have been done for centuries ever.



I always carefully check and reports of the scientist-researchers and research on fungi, pathology and mikrod activities that have been studied previously. Although much conflict of opinion about any species of fungi that can produce sandalwood or no findings were accurate or not certain fungi can produce sandalwood. From the previous research, proven, aloes produced through the process of decay, wounded and injured, occurred because infection fungal reproduction, the injured area and exposed to air, water and insect attack. Thank you.

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